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Journal: Life
Article Title: Isolation of Human Osteal Macrophages
doi: 10.3390/life16030376
Figure Lengend Snippet: Gating strategy for cell sorting of osteomacs (CD45 + ALP − CD14 + ). After Gating of single cells, using the width of the forward and side-scatter signal (FSC-W, SSC-W), living cells were selected (Zombie-Aqua-). CD45 + ALP − cells were then selected to identify the osteomac population by CD14 + . Those cells were selected for sorting. Shown here is a representative FACS run. This FACS protocol was established with n = 3 donors.
Article Snippet:
Techniques: FACS
Journal: Life
Article Title: Isolation of Human Osteal Macrophages
doi: 10.3390/life16030376
Figure Lengend Snippet: Macrophage surface marker expression of osteomacs isolated by MACS ® . The immunofluorescence (IF) images show staining for the macrophage markers CD14, CD45 and markers for M1-like (CD86 and CD68) and M2-like macrophage polarization (CD163, CD209) together with nuclei staining by using DAPI (blue). Arrows indicate thread-like configuration of some osteomacs. Shown here are representative IF images. IF staining was performed with n = 2 donors.
Article Snippet:
Techniques: Marker, Expressing, Isolation, Immunofluorescence, Staining
Journal: JCI Insight
Article Title: A platelet transcriptomic signature of thromboinflammation predicts cardiovascular risk
doi: 10.1172/jci.insight.195824
Figure Lengend Snippet: ( A ) Using flow cytometric analysis of fixed whole blood, monocytes were identified as CD14 + cells within the CD45 + cell population. CD61 + cells within the CD14 + monocyte population were defined as monocyte-platelet aggregates (MPA). ( B ) shows MPA levels from patients with available MPA levels at both time points (TP). High MPA is defined as ≥ 14.1% and low MPA as ≤ 11.7%.
Article Snippet: Citrate-anticoagulated whole blood was fixed with 1% formalin (Crystalgen catalog CG-190), and stained with CD61 FITC (Agilent DAKO item #F0803), CD86 PE (catalog 555665), CD40 V450 (catalog 561219) (BD),
Techniques: